Revisión documental sobre el software GeoGebra aplicado a la Cultura Física y el deporte

El software GeoGebra es muy útil en las simulaciones de movimientos físicos porque con la ayuda de este el profesional del deporte es capaz de interpretar con valores matemáticos y geométricos la realización de un lanzamiento, una acción que a simple vista es muy difícil descifrar. Sin embargo, a pesar de ser este recurso una vía recurrente para contribuir al desarrollo de la cultura física y el deporte, no ha sido muy generalizado su uso en esta área del conocimiento. Es por ello que los autores de esta investigación se proponen como objetivo: realizar una sistematización documental sobre el software Geogebra enfocado en la cultura Física para que se convierta en un referente a tener en cuenta para futuras innovaciones e investigaciones referente al tema. Con vistas a lograr esta meta consultaron un grupo de artículos en bases de datos de Scopus y Google Académico y publicados en los últimos cinco años referente al uso del Software GeoGebra y se visualizaron las características de este medio y los diversos beneficios que ha ofrecido a través del tiempo. Los artículos hallados se sistematizaron considerando la matriz de análisis, conteniendo aspectos preponderantes en cantidad de publicaciones durante el año 2021 con 12 publicaciones y menor cantidad de publicaciones durante el 2022. Se estudió un prototipo de 29 artículos, en los últimos cinco años referente al uso del Softwares educativos que implican al GeoGebra.

O software GeoGebra é muito útil na simulação de movimentos físicos porque com a ajuda dele o profissional do esporte consegue interpretar com valores matemáticos e geométricos a execução de um arremesso, ação muito difícil de decifrar a olho nu. Contudo, apesar deste recurso ser uma forma recorrente de contribuir para o desenvolvimento da cultura física e do desporto, a sua utilização nesta área do conhecimento não tem sido muito difundida. É por isso que os autores desta pesquisa propõem como objetivo: realizar uma sistematização documental sobre o software Geogebra voltado para a Cultura Física para que ele se torne uma referência a ser levada em conta para futuras inovações e pesquisas sobre o tema. do tempo. Os artigos encontrados foram sistematizados considerando a matriz de análise, contendo aspectos predominantes no número de publicações durante o ano de 2021 com 12 publicações e um menor número de publicações durante 2022. Foi estudado um protótipo de 29 artigos, nos últimos cinco anos quanto ao uso de software educacional que envolve o GeoGebra.

The GeoGebra software is very useful in simulating physical movements because with the help of it the sports professional is able to interpret with mathematical and geometric values the execution of a throw, an action that is very difficult to decipher with the naked eye. However, although this resource is a recurring way to contribute to the development of physical culture and sports, its use in this area of knowledge has not been very widespread. That is why the authors of this research propose as an objective: to carry out a documentary systematization on the Geogebra software focused on Physical culture so that it becomes a reference to be taken into account for future innovations and research on the topic. With a view to achieving this goal, they consulted a group of articles in Scopus and Google Scholar databases and published in the last five years regarding the use of GeoGebra Software and the characteristics of this medium, as well as the various benefits it has offered to users through time were visualized. The articles found were systematized considering the analysis matrix, containing predominant aspects in the number of publications during the year 2021 with 12 publications and a smaller number of publications during 2022. A prototype of 29 articles was studied, in the last five years regarding the use of Educational software that involves GeoGebra.

Comparison of the shear bond strength of new and recycled metallic brackets using different adhesive materials : an in vitro study.

Purpose: To evaluate and compare shear bond strength (SBS) of new and recycled metallic brackets bonded to conditioned and reconditioned enamel, using two different adhesive materials. Materials and methods: 72 extracted sound human premolars were randomly divided into 6 groups. Transbond XT light cured composite (LCC) and Fuji Ortho LC resin-modified glass ionomer (RMGI), were used as adhesive materials. In groups 1 and 2 (control), new brackets were bonded to sound premolars using either LCC or RMGI, respectively. In Groups 3 and 4, new brackets were bonded to reconditioned enamel; and in groups 5 and 6, sandblasted recycled brackets were rebonded to reconditioned enamel. After 5.000 thermal cycles between 5ºC and 55ºC, SBS was evaluated and adhesive remnant on the enamel assessed using the ARI index. Statistical analyses included Shapiro-Wilk, ANOVA, Fligner-Killeen ANOVA and Tukey tests. Results: The statistical analysis showed no significant difference in SBS comparing control and experimental groups for either new or recycled brackets (p = 0.848). The SBS was significantly higher in brackets bonded with LCC (15.7 MPa) than RMGI (11.6 MPa) (p = 0.006). Adhesive failure was the most frequent, with the adhesive remnant covering more than 50% of the bracket base. Conclusion: No significant differences were observed in SBS using either new or recycled brackets, regardless of the dental surface treatment (conditioned or reconditioned). Significantly higher SBS values were obtained with LCC adhesive. Adhesive failure prevails in all groups.

Characterization of Brazilian Cordyceps fumosorosea isolates: Conidial production, tolerance to ultraviolet-B radiation, and elevated temperature.

Cordyceps fumosorosea is an entomopathogenic fungus with a global distribution and is used for the biological control of agricultural pests. High conidial productivity and tolerance to abiotic stresses such as elevated temperature and ultraviolet radiation (UV-B) are desired characteristics in candidate isolates for commercial products. Our goal in this study was to characterize promising isolates of C. fumosorosea from five Brazilian biomes regarding conidial production, tolerance to UV-B, and elevated temperature (45°). Seventy-two isolates out of 172 were chosen visually, based on growth and sporulation in culture medium, and grown on parboiled rice. Next, fourteen isolates were selected, based on productivity on rice and origin of isolation, for production in polypropylene bags and submitted to UV-B for 2, 4, 6, and 8 h or to 45 °C for 30, 60, and 90 min. High variations in conidial production were observed among isolates, and a positive correlation was observed between UV-B and heat tolerance. The isolates ESALQ4556 and ESALQ4778 showed the highest yields of conidial production in polypropylene bags (3.51 × 109 conidia/g dry rice), while ESALQ1296, an isolate recovered from insects, was the most tolerant to UV-B and 45 °C. Exposure to radiation for more than 4 h and placed directly at 45 °C for more than 30 min significantly reduced conidial germination for all C. fumosorosea isolates. These results contribute to a better understanding of the tolerance to abiotic factors of Brazilian isolates of C. fumosorosea.

Crisis adrenal neonatal secundaria a hipoplasia hipofisaria / Neonatal Adrenal Crisis Secondary to Pituitary Hypoplasia

Introducción: La insuficiencia adrenal hipotálamo hipofisaria usualmente se manifiesta secundaria a tumores y, cuando resulta congénita se asocia, con frecuencia, con otras deficiencias hormonales. La crisis adrenal suele presentarse en su debut y puede resultar potencialmente mortal. Objetivo: Examinar el caso de una paciente con insuficiencia adrenal central que debutó con una crisis adrenal congénita. Presentación del caso: Recién nacida a término, padres no consanguíneos, hospitalizada a los 9 días de vida por clínica de una semana con múltiples episodios eméticos y apnea. Ingresó con deshidratación severa, hipotensa y estuporosa. Además, se encontró acidosis metabólica severa, hipoglucemia persistente, hiponatremia e insuficiencia prerrenal. Ante la no mejoría de su estado hemodinámico, a pesar del uso de cristaloides y vasopresores, finalmente mejoró con la administración de dosis altas de hidrocortisona. El diagnóstico de deficiencia de cortisol de origen central se realizó con un test dinámico de insulina y la resonancia magnética nuclear hipofisaria. Conclusiones: La crisis adrenal se debe tener presente como diagnóstico diferencial en episodios agudos con inestabilidad hemodinámica persistente e hipoglucemia de difícil manejo. Adicionalmente, hay que considerar que existen otras causas menos comunes de insuficiencia adrenal en neonatos como la hipoplasia hipofisaria(AU)

Introduction: Hypothalamic-pituitary adrenal insufficiency usually manifests secondary to tumors and, when congenital, is often associated with other hormonal deficiencies. Adrenal crisis usually occurs at its onset and can be life threatening. Objective: To review the case of a patient with central adrenal insufficiency who had an onset with a congenital adrenal crisis. Case presentation: Term newborn, non-consanguineous parents, hospitalized at 9 days of life for a week-long clinical presentation with multiple emetic episodes and apnea. She was admitted with severe dehydration, hypotensive and stuporous. In addition, severe metabolic acidosis, persistent hypoglycemia, hyponatremia and prerenal failure were found. Given the lack of improvement of her hemodynamic status, despite the use of crystalloids and vasopressors, she finally improved with the administration of high doses of hydrocortisone. The diagnosis of cortisol deficiency of central origin was made with a dynamic insulin test and pituitary nuclear magnetic resonance imaging. Conclusions: Adrenal crisis should be kept in mind as a differential diagnosis in acute episodes with persistent hemodynamic instability and difficult-to-manage hypoglycemia. Additionally, other less common causes of adrenal insufficiency in neonates, such as pituitary hypoplasia, should be considered(AU)

Persistent neonatal hypoglycemia secondary to hyperinsulinism/hyperammonemia. Case report

Introduction: Hyperinsulinism/hyperammonemia syndrome (HI/HA) is a rare genetic disease caused by the activation of mutations in the GLUD1 gene. It is characterized by recurrent symptomatic hypoglycemic episodes, poor tolerance to fasting, and requirement for high metabolic fluxes of glucose, with an insulin/ glucose ratio ≥0.3. Case presentation: Preterm male newborn (36 2/7 weeks of gestation) who was delivered by caesarean section due to acute fetal distress. At birth, the patient presented with weak cry, hypotonia, mild respiratory distress, and recurrent episodes of hypoglycemia, thus 10% dextrose and hydrocortisone were administered initially. Treatment with octeoctride was started, but due to the patient's poor response, laboratory tests were performed, reporting the following findings: serum ammonia: 137.6, insulin: 39.1 µIU/mL, blood glucose: 26.06 mg/dL, and insulin/blood glucose ratio: 1.5, leading to the diagnosis of HI/HA syndrome. Treatment with diazoxide was initiated, achieving a progressive improvement in blood glucose levels; however, afterwards, he presented seizures, so midazolam, phenobarbital and valproic acid were added to the treatment regimen. When hypoglycemia and seizure episodes resolved, the patient was discharged at 2 months and 5 days of life, and a treatment based on oral administration of diazoxide, phenobarbital and valproic acid was prescribed. Conclusion: HI/HA syndrome is characterized by recurrent episodes of hypoglycemia and hyperammonemia; therefore, the presence of these two conditions in neonates is highly suggestive of the disease. Timely diagnosis and treatment are required to avoid neurological sequelae, and transdisciplinary assessment is of great importance, as it increases the likelihood of early diagnosis and timely administration of diazoxide to restore normal glucose levels.

Introducción. El síndrome hiperinsulinismo/hiperamoniemia (HI/HA) es una enfermedad genética rara causada por la activación de mutaciones en el gen GLUD1. Este síndrome se caracteriza por hipoglucemias sintomáticas recurrentes, poca tolerancia al ayuno y requerimiento de altos flujos metabólicos de glucosa, con un índice insulina/glucosa ≥0.3. Presentación del caso. Recién nacido masculino pretérmino de 36 2/7 semanas de gestación que nació por cesárea debido a sufrimiento fetal agudo. Al nacer, el paciente presentó llanto débil, hipotonia, dificultad respiratoria leve y episodios recurrentes de hipoglicemia, por lo que inicialmente se administró dextrosa 10% e hidrocortisona. Se inició tratamiento con octeoctride, pero ante la pobre respuesta, se realizaron exámenes de laboratorio en los que se reportó lo siguiente: amonio sérico: 137.6 pmol/L, insulina: 39.1 µUl/mL, glucemia: 26.06 mg/dL y relación insulina/glucemia: 1.5, lo que permitió diagnosticarlo con síndrome HI/HA. Se inició tratamiento con diazóxido, lográndose mejora progresiva de la glicemia; pero posteriormente presentó crisis convulsivas, por lo que se agregó midazolam, fenobarbital y ácido valproico al tratamiento. Al resolver la hipoglicemia y las convulsiones, el paciente fue dado de alta a los 2 meses y 5 días de vida, prescribiéndose un tratamiento basado en la administración oral de diazóxido, fenobarbital y ácido valproico. Conclusión. El síndrome HI/HA se caracteriza por hipoglucemias recurrentes e hiperamoniemia; por tanto, la presencia de estas dos condiciones en neonatos es altamente sugestiva de la enfermedad. Su diagnóstico y tratamiento deben ser oportunos para evitar secuelas neurológicas, siendo la valoración transdisciplinaria de gran importancia, pues aumenta las probabilidades de un diagnóstico temprano y administración oportuna de diazóxido para restablecer los niveles normales de glucosa.

Tratamiento de Superficie utilizados en Microtornillos de Ortodoncia y su Efecto en la Estabilidad Primaria y Secundaria: Revisión Bibliográfica

Resumen: Los microtornillos de ortodoncia corresponden a dispositivos de anclaje temporal que sirven como coadyuvantes en el tratamiento ortodóncico y cuyo uso se ha ido incrementando en el último tiempo. Las fallas de los microtornillos tienden a ocurrir durante la primera semana de inserción por lo que mejorar la estabilidad es un paso importante para mejorar la confiabilidad del tratamiento. Una posible solución a esto es modificar la superficie del microtornillo. Objetivo: Identificar en la literatura actual los tratamientos de superficie más utilizados que favorezcan la estabilidad primaria y secundaria en el éxito de los microtornillos en ortodoncia. Material y método: Se realizó una búsqueda en Pubmed y EBSCO con los términos en idioma inglés "miniscrew"/"mini implant" AND "surface", "treatment" and "stability". Se incluyeron los estudios realizados in vivo con el objetivo de comparar y/o evaluar el efecto de los tratamientos realizados en la superficie del microtornillo en el éxito o estabilidad de éste, artículos disponibles en inglés y español. Se excluyeron estudios realizados en implantes dentales y/o médicos, in vitro, estudios clínicos sin grupo control. Resultados: 25 publicaciones fueron utilizadas en la revisión, habiendo 11 tipos de tratamiento de superficie estudiados. La generación de ma trices de nanotubos de óxido de titanio, fotofuncionalización mediada por rayos ultravioleta y anodizado de superficie evidenciaron aumento de la estabilidad de los microtornillos. El uso de técnicas convencionales: grabado ácido, arenado-grabado ácido, no es concluyente en cuanto a su efecto en la estabilidad. Conclusión: Hay escasa evidencia sobre los tratamientos de superficie realizados en microtornillos de ortodoncia para la mejora de su estabilidad. Técnicas pioneras como la generación de matrices de nanotubos de óxido de titanio, fotofuncionalización mediada por rayos ultravioleta y anodizado de superficie evidenciaron aumento de la estabilidad de los microtornillos, siendo necesaria la replicación de los estudios en humanos. La utilización de técnicas convencionales tales como grabado ácido y arenado-grabado ácido, no es concluyente en cuanto a su efecto en la estabilidad de los microtornillos.

Abstract: Orthodontic miniscrews are temporary anchoring devices that help as adjuvants in orthodontic treatment and whose use has increased in recent times. Miniscrew failures can happen during the first week of insertion, so improving stability is an important step to enhance treatment reliability. A possible solution to this issue is to modify the miniscrew surface. Objective: To identify in the current literature the most widely used surface treatments that favor the primary and secondary stability and success of orthodontic miniscrews. Material and method: A search was made in Pubmed and EBSCO with the English terms "miniscrew"/"mini implant" AND "surface", "treatment" and "stability". In vivo studies were included with the aim of comparing and/or evaluating the effect of the treatments performed on the miniscrew's surface and their success or stability, articles available in English and Spanish. Studies performed in dental and/or medical implants, in vitro, clinical studies without control group were excluded. Results: 25 publications were used in the review, with 11 types of surface treatment studied. The generation of titanium oxide nanotube matrices, ultraviolet-mediated photofunctionalization and surface anodizing showed an increase in the stability of the miniscrews. The use of conventional techniques: acid etching, sandblasting-acid etching, is inconclusive as to its effect on stability. Conclusion: There is little evidence of surface treatments performed on orthodontic miniscrews to improve their stability. Pioneering techniques such as the generation of titanium oxide nanotube matrices, ultraviolet-mediated photofunctionalization and surface anodizing showed increased stability of the miniscrews, and require their replication on human studies. The use of conventional techniques such as acid etching and acid sandblasting-etching is inconclusive as to its effect on the stability of the miniscrews.

Comparison of shear bond strength of metal orthodontic brackets bonded to a CAD/CAM prosthetic provisional material after the use of a self-adhesive resin cement versus a light adhesive paste and different surface conditioning protocols: An in vitro study.

BACKGROUND: Adult orthodontic treatment has been increasingly popular, and brackets may need to be bonded to provisional crowns, including CAD/CAM crowns. The use of self-adhesive resin cement or light adhesive paste have been suggested with different surface conditioning protocols to improve the adhesion to CAD/CAM PMMA provisional crowns. Objective To determine and compare the in vitro shear bond strength (SBS) of metal brackets bonded to a provisional prosthetic CAD/CAM material after the use of different adhesive cements and surface conditioning protocols. MATERIALS AND METHODS: One hundred twenty Telio® CAD specimens were manufactured in 12 groups (n=10). Each specimen was bonded to a metal bracket and divided according to adhesive technique (3M™Transbond™ XT Light Cure Paste or 3M™RelyX™ U200) surface treatment (macroretentions) and the use of silane. Half of the specimens were thermocycled (5000 cycles, 5°C/55°C water baths). The SBS test was carried out using a shear bond strength tester, and the type of adhesive failure was determined by means of the adhesive remnant index. The data were analysed with the Mann-Whitney test (α=0.05). RESULTS: Statistically significant differences (P<0.001) in SBS were found among the groups. The group with macroretentions, silane, and not thermocycled was the one that obtained the highest average value (17.31±4.89MPa). The lowest average value was the group without macroretentions, without silane, and thermocycled (3.4±3.37MPa). CONCLUSION: The shear bond strength of brackets to provisional prosthetic CAD/CAM materials depended on the type of adhesive, surface treatment, and aging by thermocycling.

¿Qué saben de pubertad, relaciones de pareja y reproducción, un grupo de jóvenes chilenos con Síndrome de Down? / What do a Group of Young Chilean with Down Syndrome Know about Puberty, Relationshipsand Reproduction?

Introducción: vivir la sexualidad y afectividad de forma plena es fundamental en todo ser humano; sin embargo, las personas con síndrome de Down (SD) tienen escasas instancias de educación sexual y pocos espacios de socialización para experimentarla. Objetivos: describir el conocimiento que tienen jóvenes con SD sobre temáticas básicas de pubertad, relaciones de pareja y reproducción. Metodología: estudio cualitativo, descriptivo, exploratorio, en dos grupos focales de jóvenes con SD, entre 13 y 18 años, separados por género. La información fue grabada, transcrita y codificada según categorías emergentes. Resultados: sobre la palabra “sexualidad”, las mujeresdeclararon no haberla escuchado y los hombres hicieron referencia a “sexo”, “relaciones de pareja”, “amor” y “familia”. Para “cambios puberales”, ambos grupos señalaron los cambios más visibles, pero nada respecto a reproducción. Solo un participante entendía el concepto de “relación sexual”. Las mujeres consideraron el “inicio de la vida humana” como un evento de generación espontánea y los hombres plantearon una teoría fantasiosa. La familia fue la principal fuente de información. Discusión: los participantes mostraron un manejo deficiente e infantilizado de la información. Son urgentes la investigación y el desarrollo de programas de Educación Sexual y así dignificar la sexualidad y afectividad de personas con SD, desde un enfoque de derechos. (AU)

Introduction: living sexuality and affectivity fully is fundamental in every human being, however, people with Down syndrome (DS) have few instances of sex-ual education and few spaces for socialization to experience it. Objectives: describe the knowledge that young people with DS have on basic issues of puberty, relationships and reproduction. Methodology: qualitative, descriptive, exploratory study in two focus groups of young people with DS, between 13 and 18 years old, separated by gender. The information was recorded, coded according to categories. Results: regarding the word “sexuality”, the women declared that they had not heard it and the men referred to “sex”, “couple relationships”, “love” and “family”. For “pubertal changes”, both groups reported the most visible changes, but nothing regarding reproduction. Only one participant understood the concept of “sexual relationship”. Women considered the “beginning of human life” as a spontaneous generation event and the men came up with a fanciful theory. The family was the main source of information. Discussion: par-ticipants showed deficient and infantilized handling of information. Research and devel-opment of Sex Education programs are urgent to dignify the sexuality and affectivity of people with DS, from a rights’ perspective. (AU)

Comparative study of molar and incisor bite forces regarding deciduous, mixed, and definitive dentition.

Objective: To compare maximum incisor (MBFinc) and molar (MBFmol) bite forces regarding the type of dentition and sex and to establish a relationship between them.Methods: One hundred-five individuals were divided into 3 groups: G1 - 22 females/13 males (4-5 years); G2 - 15 females/20 males (11-12 years); and G3 - 16 females/19 males (17- 18 years). The maximum bite force was recorded with an extraoral measuring device. Three measurements were recorded: right and left molar level (MBFmol) and incisor level (MBFinc). The ratio %MBFinc/MBFmol was determined.Results: MBFmol and MBFinc values increased with age (p < 0.05). MBFmol showed statistically higher values than MBFinc (p < 0.05), when compared within the same group.Conclusion: MBFinc and MBFmol increased with age. For all groups, MBFmol showed higher values than MBFinc, and %MBFinc/MBFmol ratio was 1:2. No differences were found in maximum bite force between sexes.

45,X/46,XY Mosaicism with Male Phenotype: Case Report.

Mixed gonadal dysgenesis is the most common chromosomal abnormality with ambiguous genitalia, defined as a 45,X/46,XY mosaicism. It can present with a normal male phenotype, ambiguous genitalia, or features of Turner syndrome. A 14-year-old patient was referred to the genetics clinic due to hypospadia, cryptorchidism, and aortic coarctation. During the physical examination, short stature, webbed neck, and Blashko lines on his back were noted. He had a previous karyotype reported as normal. However, due to an inadequate evolution and a low resolution on the previous test, a higher resolution karyotype was performed, identifying a mosaicism 45,X/46,XY. A multidisciplinary board examined the case, and follow-up with tumor markers was carried out to evaluate the presence of gonadoblastoma, one of the main complications in these patients. Treatment should be transdisciplinary and focused on the particular characteristics of each case. Other treatment alternatives include corrective surgery and hormonal therapy.

Ausencia congénita de incisivos laterales superiores. Enfoques terapéuticos y sus resultados a largo plazo. Revisión descriptiva

RESUMEN: La agenesia de incisivos laterales superiores corresponde a un desafío clínico, en cuanto a planificación de tratamiento, así como también para la obtención de resultados estéticos y funcionales adecuados. Con el fin de conseguirlo, el clínico debería informarse sobre las diferentes opciones terapéuticas disponibles en la actualidad, considerando que la indicación depende de cada caso clínico individual, es decir, del paciente en su contexto integral sumado a su condición intra-oral. Además, es importante evaluar los resultados a largo plazo de las diferentes alternativas, para lograr la resolución clínica más adecuada y estable en el tiempo. El éxito en estos pacientes, requiere de un enfoque multidisciplinario, considerando especialistas en ortodoncia, rehabilitación oral, periodoncia y cirugía oral de ser necesario. El objetivo de esta revisión es describir y comparar las alternativas terapéuticas para la agenesia de incisivos laterales maxilares.

ABSTRACT: The agenesis of upper lateral incisors represents a clinical challenge regarding treatment planning and appropriate aesthetic and functional results. In order to obtain the best results, the clinician should be informed about the different therapeutic options currently available, considering that the indication depends on each individual clinical case, that is, on the patient in his/her comprehensive context in addition to his/her oral condition. It is also important to evaluate long term results from each treatment available, in order to achieve the better outcome and most importantly, a stable clinical resolution over time. The treatment success in this kind of patients requires a multidisciplinary approach, including specialists in orthodontics, oral rehabilitation, periodontics, and oral surgery if necessary. The objective of this review is to describe and compare the therapeutic alternatives for maxillary lateral incisor agenesis.

Análisis comparativo in vitro de la resistencia adhesiva al cizallamiento de brackets metálicos adheridos a superficies dentarias tratadas con diferentes agentes blanqueadores / Comparative in vitro analysis of the adhesive resistance to the shear bond strength of metal brackets adhered to dental surfaces treated with different bleaching agents

RESUMEN: Objetivo: Evaluar y comparar el efecto de tres agentes blanqueadores en la resistencia adhesiva al cizallamiento de brackets metálicos. Método: Se cementaron 76 brackets en premolares humanos extraídos por indicación de ortodoncia. Se establecieron 4 grupos: (0) control, (1) esmalte tratado con Peróxido de Carbamida al 16%, (2) Peróxido de Hidrógeno al 35% y (3) tiras de blanqueamiento con Peróxido de Hidrógeno al 10%. Los brackets fueron cementados con Transbond XT (3M Unitek, EE.UU.). Se realizaron 5.000 ciclos de termociclado, luego se evaluó la resistencia adhesiva mediante una máquina Bisco y la cantidad de material adhesivo remanente según el índice adhesivo remanente (ARI). El análisis estadístico incluyó pruebas de Shapiro- Wilk, Kruskal -Wallis y Mann-Whitney. Resultados: Los grupos 0, 1, 2, 3 obtuvieron fuerzas de resistencia de 12.64, 11.06, 6.4 y 9.34 Mpa respectivamente con diferencia estadística entre los grupos (p <0.05). El ARI fue significativamente diferente en algunos grupos (p <0.05), indicando fallas adhesivas para el grupo 2 (p <0.05), cohesivas para el grupo control y de ambos tipos para los grupos 1 y 3. Conclusiones: El peróxido de hidrógeno al 35% reduce la resistencia adhesiva al cizallamiento en brackets metálicos sometidos a termociclado 24 horas luego del blanqueamiento.

ABSTRACT: Aim: To evaluate and compare the effect of three bleaching agents on the shear bond strength of metallic brackets. Method: Brackets were cemented on 76 human premolars extracted for orthodontic reasons. 4 groups were evaluated: (0) Control, (1) enamel treated with Carbamide Peroxide 16%, (2) Hydrogen Peroxide 35% and (3) Hydrogen Peroxide 10% "Whitening strips". All brackets were cemented using Transbond XT adhesive system (3M, Unitek, USA). All samples were thermocycled (5.000 cycles). Shear bond strength was evaluated with a Bisco machine and the amount of adhesive material at the base of the brackets and enamel surface was assessed using the adhesive remnant index (ARI). The statistical analysis included Shapiro-Wilk, Kruskal-Wallis and Mann-Whitney U tests. Results: The results of shear bond strength for groups 0, 1, 2, and 3 were 12.64, 11.06, 6.4 and 9.34 MPa respectively, with statistically significant differences between groups (p <0.05). ARI index was significantly different in some groups (p <0.05), indicating cohesive failure for control, adhesive failure for group 2 (p <0.05), adhesive and cohesive failure for groups 1 and 3. Conclusions: The 35% hydrogen peroxide office bleaching agent reduces adhesion values in the metal brackets thermocycled 24 hours after bleaching.

Transcriptional profiles along cell programming into corneal epithelial differentiation.

Using the rabbit corneal epithelial cell line RCE1(5T5) as a model, we analyzed three differentiation stages, distinguished on basis to the growth state of cultured cells and after studying the expression of transcription factors such as Oct4, Pax6 and ΔNp63α, selected differentiation markers, and signaling or epigenetic markers such as Notch receptors and Prdm3. Namely, proliferative non-differentiated cells, committed cells, and cells that constitute a stratified epithelium with a limbal epithelial-like structure. RNAseq based transcriptome analysis showed that 4891 genes were differentially expressed among these stages displaying distinctive gene signatures: proliferative cells had 1278 genes as gene signature, and seem to be early epithelial progenitors with an Oct4+, KLF4+, Myc+, ΔNp63α+, ABCG2+, Vimentin+, Zeb1+, VANGL1+, Krt3-, Krt12- phenotype. Committed cells had a gene signature with 417 genes and displayed markers indicative of the beginning of corneal differentiation, and genes characteristic of proliferative cells; we found the possible participation of Six3 and Six4 transcription factors along this stage. The third stage matches with a stratified corneal epithelium (gene signature comprising 979 genes) and is typified by an increase in the expression of WNT10A and NOTCH 2 and 3 signaling and Cux1 transcription factor, besides Pax6, KLF4 or Sox9. The differentiated cells express about 50% of the genes that belong to the Epidermal Differentiation Complex (EDC). Analysis of the differences between corneal epithelium and epidermis could be crucial to understand the regulatory mechanisms that lead to the expression of the differentiated phenotype.

Comparison of the primary stability of orthodontic miniscrews after repeated insertion cycles.

OBJECTIVES: To compare the primary stability of miniscrews after repeated cycles of insertion through insertion torque (IT) measurements and resonance frequency analysis (RFA). MATERIALS AND METHODS: Sixty titanium miniscrews were divided into two groups according to the insertion protocol: one with predrilled sites and the other self-drilled into porcine iliac crest bone specimens. Each group had three cycles of reinsertion. After each insertion, the IT and RFA were measured. The IT was measured by using a torque meter, and the RFA was measured using the Osstell ISQ device. A total of five miniscrews of each group were selected for sequential assessment of the morphology of their tip and threads using scanning electron microscopy after each insertion cycle. RESULTS: No statistically significant differences were found in the IT values of miniscrews reinserted up to three times in the group with predrilled surgical sites. The IT value increased significantly with the number of reinsertions in the self-drilled group. The RFA value decreased as the number of insertions increased in both groups. CONCLUSIONS: Under the conditions of this in vitro study, reinserting miniscrews deteriorates the integrity of their tip and thread. Reinsertion should be discouraged particularly when insertion sites are not predrilled.

Shear bond strength evaluation of metallic brackets bonded to a CAD/CAM PMMA material compared to traditional prosthetic temporary materials: an in vitro study.

INTRODUCTION: Orthodontic treatment for adults is currently increasing, and therefore the need to bond brackets to restorations and temporary crowns. The use of CAD/CAM PMMA provisional restorations for orthodontic purposes have not yet been described, and there is currently insufficient information regarding the strength of bracket adhesion. OBJECTIVE: This study aimed at evaluating the effects of thermocycling (TC) and surface treatment on shear bond strength (SBS) of brackets to different provisional materials. METHODS: Forty specimens were made from each material [PMMA (Telio Lab), bis-acryl (Telio CS C&B), and PMMA CAD/CAM (Telio CAD)], sandpapered, and divided according to surface treatment (pumiced or sandblasted) and TC (half of the samples = 1,000 cycles, 5°C/55°C water baths) (n = 10/group). Stainless-steel brackets were bonded to the specimens (using Transbond XT), and SBS testing was performed. Data were analyzed by three-way ANOVA and LSD post-hoc tests (α = 0.05). Failure types were classified with adhesive remnant index (ARI) scores. RESULTS: SBS values ranged from 1.5 to 14.9 MPa. Sandblasted bis-acryl and sandblasted auto-curing PMMA groups presented similar values (p> 0.05), higher than the CAD/CAM material (p< 0.05), with or without TC. When thermocycled, pumiced bis-acryl showed higher SBS than pumiced acrylic (p= 0.005) and CAD/CAM materials (p= 0.000), with statistical difference (p= 0.009). TC showed negative effect (p< 0.05) for sandblasted bis-acryl and pumiced acrylic groups. ARI predominant score was mostly zero (0) for CAD/CAM, 1 and 2 for bis-acryl, and 1 for acrylic groups. CONCLUSION: In general, bis-acryl material showed the highest SBS values, followed by acrylic and CAD/CAM materials, which showed SBS values lower than an optimum strength for bonding brackets.

Efecto del entrenamiento muscular masticatorio en el desarrollo del patrón vertical facial en niños: revisión narrativa / Training effect of the masticatory muscles in the development of the vertical facial pattern in children: narrative review

RESUMEN: Introducción: El patrón de crecimiento facial se establece antes de la aparición del primer molar permanente. Un exceso de crecimiento vertical facial, corresponde a individuos de cara larga, con ángulos goniacos abiertos, alturas faciales inferiores aumentadas y músculos masticatorios de menor área transversal. Esto se asocia a presentar mordida abierta anterior, mordida cruzada, clase II esquelético, entre otros. El entrenamiento muscular masticatorio podría controlar el excesivo crecimiento vertical, generando fuerzas opuestas que favorezcan un crecimiento horizontal. Objetivo: Describir el efecto del entrenamiento muscular masticatorio en el desarrollo del patrón vertical facial en niños. Método: Se realizó una revisión narrativa mediante búsqueda electrónica en las bases de datos PubMed, EBSCO, Scopus, Cochrane, Trip Database y Epistemonikos. Se utilizaron las palabras clave: "músculos masticatorios", "maseteros", "temporales", "masticación", "ejercicio", "entrenamiento", "crecimiento vertical", "patrón vertical", "dolicofacial", "control vertical". Los términos MeSH: "músculos masticatorios", "ejercicio". Resultados: Se seleccionaron 15 artículos de los cuales 9 son ensayos clínicos, 4 son reportes de caso y 2 son estudios observacionales transversales. Conclusiones: El entrenamiento muscular masticatorio tiene efectos positivos, favoreciendo un mayor crecimiento horizontal en niños con patrón vertical. No obstante, faltan estudios y ensayos clínicos para establecer y cuantificar los cambios morfológicos generados por el entrenamiento muscular.

ABSTRACT: Background: The pattern of facial growth is established before the eruption of the first permanent molar. An excess of vertical facial growth corresponds to individuals with long faces, open gonial angles, higher inferior facial heights and smaller masticatory muscles associated with different dental anomalies like anterior open bite, Brodie bite, skeletal class II and others. The masticatory muscle training could control the excessive growth in the vertical dimension, making opposing forces that will favor the horizontal growth in the patient. Objective: Describe the effect of the masticatory muscle training in the development of the vertical pattern in children. Method: A narrative review was done by an electronic research in PubMed, EBSCO, Scopus, Cochrane, Trip Database y Epistemonikos. The following key words were used: "masticatory muscles", "masseter", "temporalis", "mastication", "chewing", "exercise", "training", "vertical growth", "vertical pattern", "dolichofacial", "vertical control". The MeSH terms: "masticatory muscles", "exercise". Results: Fifteen articles were selected, 9 of them were clinical trials, 4 were case reports and 2 were observational studies. Conclusions: The literature review shows that the masticatory muscle training has positive effects, favoring the horizontal growth in children with vertical facial pattern. Nevertheless, there is a lack of studies and clinical trials, that could help us to establish and quantify the morphological changes made by the masticatory muscle training.

Shear bond strength evaluation of metallic brackets bonded to a CAD/CAM PMMA material compared to traditional prosthetic temporary materials: an in vitro study

ABSTRACT Introduction: Orthodontic treatment for adults is currently increasing, and therefore the need to bond brackets to restorations and temporary crowns. The use of CAD/CAM PMMA provisional restorations for orthodontic purposes have not yet been described, and there is currently insufficient information regarding the strength of bracket adhesion. Objective: This study aimed at evaluating the effects of thermocycling (TC) and surface treatment on shear bond strength (SBS) of brackets to different provisional materials. Methods: Forty specimens were made from each material [PMMA (Telio Lab), bis-acryl (Telio CS C&B), and PMMA CAD/CAM (Telio CAD)], sandpapered, and divided according to surface treatment (pumiced or sandblasted) and TC (half of the samples = 1,000 cycles, 5°C/55°C water baths) (n = 10/group). Stainless-steel brackets were bonded to the specimens (using Transbond XT), and SBS testing was performed. Data were analyzed by three-way ANOVA and LSD post-hoc tests (α = 0.05). Failure types were classified with adhesive remnant index (ARI) scores. Results: SBS values ranged from 1.5 to 14.9 MPa. Sandblasted bis-acryl and sandblasted auto-curing PMMA groups presented similar values (p> 0.05), higher than the CAD/CAM material (p< 0.05), with or without TC. When thermocycled, pumiced bis-acryl showed higher SBS than pumiced acrylic (p= 0.005) and CAD/CAM materials (p= 0.000), with statistical difference (p= 0.009). TC showed negative effect (p< 0.05) for sandblasted bis-acryl and pumiced acrylic groups. ARI predominant score was mostly zero (0) for CAD/CAM, 1 and 2 for bis-acryl, and 1 for acrylic groups. Conclusion: In general, bis-acryl material showed the highest SBS values, followed by acrylic and CAD/CAM materials, which showed SBS values lower than an optimum strength for bonding brackets.

RESUMO Introdução: Atualmente, a demanda por tratamento ortodôntico em adultos tem aumentado. Consequentemente, também tem aumentado a necessidade de se colar braquetes em restaurações e coroas provisórias. O uso de restaurações provisórias de PMMA CAD/CAM com finalidade ortodôntica ainda não foi descrito e, até a presente data, não há informação suficiente sobre a resistência da colagem dos braquetes a esse tipo de material. Objetivo: O presente estudo teve como objetivo avaliar os efeitos da termociclagem (TC) e do tratamento de superfície sobre a resistência da colagem ao cisalhamento (RC) de braquetes colados em diferentes materiais provisórios. Métodos: Quarenta espécimes foram confeccionados de cada material: resina acrílica PMMA (Telio Lab), resina bisacrílica (Telio CS C&B), e PMMA CAD/CAM (Telio CAD). Em seguida, foram lixados e divididos de acordo com o tratamento de superfície (polidos ou jateados) e TC (metade da amostra - 1.000 ciclos de imersão em água a 5°C e 55°C) (n = 10/grupo). Braquetes de aço inoxidável foram colados aos espécimes (utilizando Transbond XT) e realizou-se o teste de RC. As informações foram analisadas por meio dos testes ANOVA de três vias e post-hoc LSD (α?#8197;= 0,05). Os tipos de falha foram classificados de acordo com os escores do índice de adesivo remanescente (ARI). Resultados: Os valores de RC variaram de 1,5 a 14,9 MPa. Os grupos de resina bisacrílica jateada e de PMMA autopolimerizável jateado apresentaram valores semelhantes (p> 0,05), superiores ao do material CAD/CAM (p< 0,05) com ou sem TC. Quando submetido à TC, o grupo de resina bisacrílica polida apresentou resistência ao cisalhamento maior do que os grupos de resina acrílica polida (p= 0,005) e do material CAD/CAM (p= 0,000), com diferença estatística (p= 0,009). A TC apresentou efeito negativo (p< 0,05) para os grupos de resina bisacrílica jateada e de resina acrílica polida. O escore ARI = 0 foi predominante para o grupo CAD/CAM; os escores 1 e 2, para o grupo resina bisacrílica; e o escore 1, para o grupo resina acrílica. Conclusão: De forma geral, a resina bisacrílica apresentou os maiores valores de RC, seguida da resina acrílica e do material CAD/CAM, que apresentaram valores de RC inferiores à força recomendada para colagem de braquetes.

Precocious puberty due to congenital adrenal hyperplasia. Case report / Pubertad precoz por hiperplasia adrenal congénita. Reporte de caso

Abstract Introduction: Premature pubarche occurs in girls before the age of 8 or boys before the age of 9 and is a sign of precocious puberty associated with increased growth acceleration. Precocious puberty can be caused, among others, by nonclassic congenital adrenal hyperplasia (CAH). Case presentation: This is a case of a 4 1/2-year-old who developed premature pubarche six months before consultation, and whose parents were first-degree cousins. She had advanced bone age, her external genitalia were normal and her height was inconsistent with her mid-parental height. After performing an adrenocorticotropic hormone test (ACTH test) and other hormone tests, it was found that she had high levels of 17-hydroxyprogesterone (17-OHP), which allowed diagnosing her with nonclassic CAH. Based on this diagnosis, glucorticoid therapy was ordered, and after one year of starting the treatment she had a favorable clinical outcome and did not show any secondary sex characteristics or bone age progression. Conclusion: Nonclassic CAH is the most frequent cause of precocious puberty. Considering that this type of hyperplasia may be asymptomatic during the early days or years of life, its diagnosis must be suspected in children with precocious puberty, increased growth acceleration and advanced bone age.

Resumen Introducción. La pubarca antes de los 8 años en niñas y de los 9 años en niños, es una manifestación de la pubertad precoz asociada al aumento en la velocidad de crecimiento. La hiperplasia adrenal congénita (HAC) no clásica es una de las causas de pubertad precoz. Presentación de caso. Paciente femenina de 4 años y medio y con padres consanguíneos (primos hermanos) quien inició pubarca 6 meses antes de ser llevada a consulta. La niña presentaba edad ósea avanzada, talla discordante con la talla media parental y sus genitales externos eran normales. Luego de realizar el test de estimulación con hormona adrenocorticotropa y otros exámenes hormonales, se encontró que sus niveles de 17-hidroxiprogesterona eran elevados, lo que permitió diagnosticarla con HAC no clásica. Con base en este diagnóstico, se inició tratamiento con glucocorticoides y luego de un año de tratamiento la paciente tuvo una buena evolución clínica, ya que no se observó progresión de los caracteres sexuales secundarios ni de la edad ósea. Conclusión. La HAC no clásica es la causa más frecuente de la PPP. Ya que este tipo de hiperplasia puede ser asintomática durante los primeros días o años de vida, se debe sospechar su diagnóstico en la infancia cuando haya pubarca precoz, mayor velocidad de crecimiento y edad ósea avanzada.

Efecto del ultrasonido de baja intensidad (USBI) sobre el movimiento dentario ortodóncico: estudio in vivo en ratas Sprague-Dawley / Effect of low intensity ultrasound (USBI) in the orthodontic dental movement: in vivo study in Sprague-Dawley rats

Este estudio se realizó con el propósito de analizar el efecto de la aplicación de ondas de USBI sobre la velocidad y distancia de movimiento dentario, y su efecto a nivel histológico en ratas Sprague-Dawley. Veintitrés ratas macho Sprague-Dawley fueron parte del experimento, las que fueron divididas aleatoriamente en tres grupos. Cinco ratas fueron utilizadas como control sin movimiento dentario ortodóncico (MDO), nueve ratas fueron sometidas a una aplicación de fuerza ortodóncica sin aplicación de USBI y nueve fueron sometidas a una aplicación de fuerza ortodóncica con USBI. Estas fuerzas ortodóncicas fueron realizadas entre el aspecto mesio vestibular del primer molar superior izquierdo y el incisivo central superior, traccionando el molar hacia mesial, aplicando una fuerza aproximada de 50 gramos durante 21 días, debido a problemas con la sedación estos dos últimos grupos quedaron compuestos de seis y ocho ratas respectivamente. En el grupo MDO sin USBI, se registró una reducción del espacio entre molares e incisivos de 2 mm. En el grupo MDO con USBI dicha reducción fue de 3.4 mm, siendo esta diferencia estadísticamente significativa. Histológicamente el grupo MDO c/USBI mostró un perímetro vascular y espacios medulares aumentados respecto de los otros grupos, no obstante, no se encontraron diferencias significativas en la cantidad de osteoclastos. Fue posible observar y demostrar cuantitativamente que el USBI es una forma de estimulación mecánica eficiente para la aceleración del MDO sobre un período experimental de 21 días, donde se observaron diferencias significativas entre el grupo control, el grupo MDO sin USBI y el grupo MDO con USBI en la magnitud del movimiento dentario. Se observó una mayor vascularidad en el grupo con USBI, expresando perímetros vasculares de mayor tamaño, esto pudo deberse al efecto estimulatorio del ultrasonido sobre la angiogénesis.

The objective of this study was to analyze the effect of USBI wave application on the speed and distance of dental movement, and its effect at histological level in SpragueDawley rats. In this study twenty-three male Sprague-Dawley rats were randomly divided into three groups. Five rats were used as control without orthodontic tooth movement (MDO), nine rats were subjected to an orthodontic force application without USBI application, and nine were subjected to an orthodontic force application with USBI. The orthodontic forces were performed between the vestibular mesio aspect of the first upper left molar and upper central incisor, pulling the molar mesially, applying an approximate force of 50 grams for 21 days. In light of sedation problems these last two groups were composed of six and eight rats respectively. In the MDO group without USBI, there was a reduction in the space between molars and incisors of 2 mm. In the MDO group with USBI this reduction was 3.4mm, this difference being statistically significant. Histologically, the MDO c / USBI group showed a vascular perimeter and enlarged spinal spaces with respect to the other groups, however, no significant differences were found in the amount of osteoclasts. It was possible to quantitatively demonstrate that USBI is a form of efficient mechanical stimulation for MDO acceleration over an experimental 21-day period, where significant differences were observed in tooth movement between the control group, the group without USBI and the group with USBI. A greater vascularity was observed in the group with USBI, expressing larger vascular perimeters, which could be due to the stimulatory effect of ultrasound on angiogenesis.

Level of tumor-infiltrating lymphocytes and density of infiltrating immune cells in different malignancies.

Aim: To correlate levels of tumor-infiltrating lymphocytes (TIL) evaluated using the International Immuno-Oncology Biomarker Working Group methodology, and both density of tumor-infiltrating immune cell and clinicopathological features in different malignancies. Methods: 209 pathological samples from gastric cancer, cervical cancer (CC), non-small-lung cancer, cutaneous melanoma (CM) and glioblastoma were tested for TIL in hematoxylin eosin, and density of CD3+, CD4+, CD8+, CD20+, CD68+ and CD163+ cells by digital analysis. Results: TIL levels were higher in invasive margin compartments (IMC). TIL in IMC, intratumoral and stromal compartments predicted survival. CC and gastric cancer had higher TIL in intratumoral; CC and CM had higher TIL in stromal compartment and IMC. CM had the highest density of lymphocyte and macrophage populations. CD20 density was associated with survival in the whole series. Conclusion: Standardized evaluation of TIL levels may provide valuable prognostic information in a spectrum of different malignancies.